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Protocol for high-resolution 3D visualization of insect regenerating legs through micro-computed tomography

  • 【作者】Liang Li, Kai Cheng, Jiru Zhong, Shaojuan Zheng, Chenjing Zhao, Yejie Wen, Sheng Li, Chonghua Ren
  • 【刊名】STAR protocols
  • 【作者单位】Affiliations 1 Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, Guangzhou Key Laboratory of Insect Development Regulation and Application Research, Institute of Insect Science and Technology, School of Life Sciences, South China Normal University, Guangzhou 510631, China. 2 Department of Biology, Taiyuan Normal University, Jinzhong 030619, China. 3 Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, Guangzhou Key Laboratory of Insect Development Regulation and Application Research, Institute of Insect Science and Technology, School of Life Sciences, South China Normal University, Guangzhou 510631, China; Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510631, China; Guangmeiyuan R&D Center, Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, South China Normal University, Meizhou 514779, China. 4 Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, Guangzhou Key Laboratory of Insect Development Regulation and Application Research, Institute of Insect Science and Technology, School of Life Sciences, South China Normal University, Guangzhou 510631, China; Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510631, China; Guangmeiyuan R&D Center, Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, South China Normal University, Meizhou 514779, China. Electronic address: renchonghua111@m.scnu.edu.cn.
  • 【年份】2024
  • 【卷号】Vol.5 No.4
  • 【页码】103342
  • 【ISSN】2666-1667
  • 【关键词】Cell Biology Developmental biology Evolutionary biology Microscopy Molecular Biology. 
  • 【摘要】 Appendage regeneration occurs within the opaque exoskeleton in arthropods, making it challenging to visualize the regenerative processes dynamically. In this protocol, we present a strategy to scan and capture the high-resolution details of microstru...
  • 【文献类型】 期刊
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